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North Carolina State University
Introduction to Galaxy
July 22, 2013

**Instructor: Dave Clements

3503 Thomas Hall (The Stephens Room)
North Carolina State University, Raleigh, NC**


Registration was free, and first come, first served. The workshop is open to all members of the NCSU community. However, it is also full.


Are you a life scientist who needs to do complex analysis on large datasets?

Galaxy is an open, web-based platform for data intensive life science research that enables non-bioinformaticians to create, run, tune, and share their own bioinformatic analyses.

This hands-on workshop will teach participants how to integrate data, and perform simple and complex analysis within Galaxy. It will also cover data visualization and visual analytics, and how to share and reuse your bioinformatic analyses, all from within Galaxy.

No programming or Linux command line experience is required.


The agenda below is tentative and should be considered a work in progress.

Time Topic
9:00 Welcome
Introductions and logistics
9:20 Basic Analysis with Galaxy
Walk through a worked, hands-on example demonstrating basic analysis with Galaxy
10:40 Break
11:00 Basic Analysis into Reusable Workflows
Genericize our analysis into something we can use again.
11:30 RNA-Seq Example Part I
Review NGS data quality issues and some quality control options in Galaxy; Mapping and Splice Junction Calling with Tophat
12:30 Lunch (on your own)
1:30 RNA-Seq Example Part II
Cufflinks, Visualization, and Visual Analytics
2:20 Galaxy Project Overview
Introduction to Galaxy and the Galaxy community
2:50 Break
3:10 Sharing, Publishing, and Reproducibility with Galaxy
Share and publish analysis, datasets, and workflows with Galaxy
3:30 Setting up your own Galaxy Cluster on the Amazon Cloud
Every participant will set up their own functional and populated (but short-lived) Galaxy server on the cloud
4:45 Done


2013 Research Triangle Galaxy Workshop Tour
NCSU Department of Genetics Amazon Web Services

This workshop is generously supported by an AWS in Education grant award, and the NCSU Department of Genetics. This workshop is part of the 2013 Research Triangle Galaxy Workshop Tour.



Please distribute to any groups that might be interested. You are also encouraged to print a post a copy of the workshop flyer.


Contact [Trudy Mackay](mailto:Trudy_Mackay AT ncsu DOT edu) or [Galaxy Outreach](mailto:outreach AT galaxyproject DOT org).