University or North Carolina Chapel Hill
    Introduction to Galaxy
    July 19, 2013

    **Instructor: Dave Clements

    9:00-4:30
    Health Sciences Library Biogen-Idec Classroom (#307)
    University of North Carolina Chapel Hill**

    Registration

    Registration was free, first come first served, and open to anyone in the UNC-CH research community.

    Audience

    Are you a life scientist who needs to do complex analysis on large datasets?

    Galaxy is an open, web-based platform for data intensive life science research that enables non-bioinformaticians to create, run, tune, and share their own bioinformatic analyses.

    This hands-on workshop will teach participants how to integrate data, and perform simple and complex analysis within Galaxy. It will also cover data visualization and visual analytics, and how to share and reuse your bioinformatic analyses, all from within Galaxy.

    No programming or Linux command line experience is required.

    Agenda

    Time Topic
    9:00 Welcome
    Introductions and logistics
    9:20 Basic Analysis with Galaxy
    Walk through a worked, hands-on example demonstrating basic analysis with Galaxy
    10:40 Break
    11:00 Basic Analysis into Reusable Workflows
    Genericize our analysis into something we can use again.
    11:30 RNA-Seq Example Part I
    Review NGS data quality issues and some quality control options in Galaxy; Mapping and Splice Junction Calling with Tophat
    12:30 Lunch (on your own)
    1:30 RNA-Seq Example Part II
    Cufflinks, Visualization, and Visual Analytics
    2:20 Galaxy Project Overview
    Introduction to Galaxy and the Galaxy community
    2:40 Break
    3:00 RNA-Seq Example Part III
    Discuss and run differential gene expression analysis, and merging multiple experiments into a single picture
    3:40 Sharing, Publishing, and Reproducibility with Galaxy
    Using Galaxy for collaboration and reproducibility
    4:00 Setting up a Galaxy Cluster on the Amazon Cloud
    Introduce Amazon Web services and cloud concepts; demonstrate starting Galaxy on the cloud
    4:30 Done

    Support

    2013 Research Triangle Galaxy Workshop Tour
    UNC Health Sciences Library Amazon Web Services

    This workshop is generously supported by an AWS in Education grant award, and the UNC Health Sciences Library.

    This course will be part of both the UNC Health Sciences Library’s Basic Bioinformatics Tools Workshop series, and the 2013 Research Triangle Galaxy Workshop Tour.

    Slides

    Questions?

    Contact Barrie Hayes or Galaxy Outreach.