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Minimap2 mapper for noisy long read sequences (e.g. Nanopore, PacBio)

Do you have Nanopaore or PacBio reads? Try minimap2 as a new aligner! From minimaps homepage:

Minimap2 is a versatile sequence alignment program that aligns DNA or mRNA sequences against a large reference database. Typical use cases include:

  • mapping PacBio or Oxford Nanopore genomic reads to the human genome
  • finding overlaps between long reads with error rate up to ~15%
  • splice-aware alignment of PacBio Iso-Seq or Nanopore cDNA or Direct RNA reads against a reference genome
  • aligning Illumina single- or paired-end reads
  • assembly-to-assembly alignment
  • full-genome alignment between two closely related species with divergence below ~15%.

For ~10kb noisy reads sequences, minimap2 is tens of times faster than mainstream long-read mappers such as BLASR, BWA-MEM, NGMLR and GMAP. It is more accurate on simulated long reads and produces biologically meaningful alignment ready for downstream analyses. For >100bp Illumina short reads, minimap2 is three times as fast as BWA-MEM and Bowtie2, and as accurate on simulated data. Detailed evaluations are available from the minimap2 preprint.

Read more in the minimap2 preprint from Heng Li - Minimap2: fast pairwise alignment for long nucleotide sequences.


Marius van den Beek for developing the Galaxy integration!