Sits at http://toolshed.g2.bx.psu.edu/, is supported by the Galaxy Team, and is the main Tool Shed of choice.
Test Tool Shed is made for breaking. If you have some tools you want to try out first before making them public in the Main Tool Shed this is the place to go. Test Tool Shed is at http://testtoolshed.g2.bx.psu.edu/.
Tool Shed backing the Galaxy@PRABI server. DEfines bioinformatics tools developed by the research teams working in the perimeter of the PRABI core facility, including kissplice/kissDE, TETools, SexDetector, and priam.
The Dutch Techcentre for Life Sciences (DTL) has made its Galaxy ToolShed publicly available. The DTL ToolShed has almost 70 tools in it, from ANNOVAR to VCF-2-VariantList. This ToolShed was originally started at NBIC.