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July 22, 2013
**Instructor: Dave Clements
9:00-4:45
3503 Thomas Hall (The Stephens Room)
North Carolina State University, Raleigh, NC**
Registration
Registration was free, and first come, first served. The workshop is open to all members of the NCSU community. However, it is also full.
Audience
Are you a life scientist who needs to do complex analysis on large datasets?
Galaxy is an open, web-based platform for data intensive life science research that enables non-bioinformaticians to create, run, tune, and share their own bioinformatic analyses.
This hands-on workshop will teach participants how to integrate data, and perform simple and complex analysis within Galaxy. It will also cover data visualization and visual analytics, and how to share and reuse your bioinformatic analyses, all from within Galaxy.
No programming or Linux command line experience is required.
Agenda
The agenda below is tentative and should be considered a work in progress.
| Time | Topic |
|---|---|
| 9:00 | Welcome Introductions and logistics |
| 9:20 | Basic Analysis with Galaxy Walk through a worked, hands-on example demonstrating basic analysis with Galaxy |
| 10:40 | Break |
| 11:00 | Basic Analysis into Reusable Workflows Genericize our analysis into something we can use again. |
| 11:30 | RNA-Seq Example Part I Review NGS data quality issues and some quality control options in Galaxy; Mapping and Splice Junction Calling with Tophat |
| 12:30 | Lunch (on your own) |
| 1:30 | RNA-Seq Example Part II Cufflinks, Visualization, and Visual Analytics |
| 2:20 | Galaxy Project Overview Introduction to Galaxy and the Galaxy community |
| 2:50 | Break |
| 3:10 | Sharing, Publishing, and Reproducibility with Galaxy Share and publish analysis, datasets, and workflows with Galaxy |
| 3:30 | Setting up your own Galaxy Cluster on the Amazon Cloud Every participant will set up their own functional and populated (but short-lived) Galaxy server on the cloud |
| 4:45 | Done |
Support
This workshop is generously supported by an AWS in Education grant award, and the NCSU Department of Genetics. This workshop is part of the 2013 Research Triangle Galaxy Workshop Tour.
Slides
Flyer
Questions?
Contact [Trudy Mackay](mailto:Trudy_Mackay AT ncsu DOT edu) or [Galaxy Outreach](mailto:outreach AT galaxyproject DOT org).