Welcome to the September 2013 Galaxy Update, a monthly summary of what is going on in the Galaxy community. Galaxy Updates complement the Galaxy Development News Briefs which accompany new Galaxy releases and focus on Galaxy code updates.
New Public Servers
A record five new servers joined the list of over 40 publicly accessible Galaxy servers in August.
Galaxy-P is a multiple 'omics' data analysis platform with particular emphasis on mass spectrometry based proteomics. Galaxy-P is developed at the University of Minnesota, deployed at the Minnesota Supercomputing Institute. Pending imminent hardware upgrades, usegalaxyp.org is running with fairly limited computational resources and on disk that is not backed up. Galaxy-P is under active development - things will break and things will change, so your patience is requested.
NICTA Bioinformatics' Genome-wide Interaction Search (GWIS) for multivariate GWAS analysis cuts the computational time for analyzing all pairs of SNP interactions in genome-wide association studies (GWAS) from months to minutes on commodity computers. Any non-commercial user can request an account [via email](mailto:gwis AT nicta DOT com DOT au).
- Benjamin Goudey, David Rawlinson, Qiao Wang, Fan Shi, Herman Ferra, Richard M Campbell, Linda Stern, Michael T Inouye, Cheng Soon Ong, Adam Kowalczyk (2013), "GWIS – model-free, fast and exhaustive search for epistatic interactions in case-control GWAS", BMC Genomics, 14(Suppl 3):S10
- H37: GWIS: Online exhaustive bivariate GWAS in minutes, poster presented by David Rawlinson, et al. at ISMB 2013.
Martin Luther University Halle-Wittenberg
Martin Luther University Halle-Wittenberg's Galaxy server provides implementations of tools developed by the groups of Bioinformatics and Pattern Recognition and/or Bioinformatics in collaboration with internal and external partners.
- CRISPRer is a tool for genome-wide selection and assessment of CRISPR/Cas protospacers.
- TALgetter allows you to scan input sequences for putative target sites of a given TAL (transcription activator-like) effector as typically expressed by many Xanthomonas bacteria.
- TALgetterLong allows you to scan input sequences for putative target sites of a given TAL (transcription activator-like) effector as typically expressed by many Xanthomonas bacteria in large input data, but lacks some of the features of TALgetter (e.g., computation of empirical p-values).
- TALENoffer is a tool for predicting off-targets of TAL effector nucleases (TALENs).
- Dimont (beta) is a universal tool for de-novo motif discovery. Dimont has successfully been applied to ChIP-seq, ChIP-exo and protein-binding microarray (PBM) data.
MBAC Metabiome Portal
The MBAC Metabiome Portal is a flexible and customizable webserver that simplifies control, usage, access, and analysis of microbiome, metabolome, and immunome data (the Metabiome). The Portal uses a relational database management system and distributed analytical resources and includes several tools such as sequence clustering, filtering sequencing artifacts, taxonomic analysis, and functional annotation.
Users access the MBAC Metabiome Portal through a Galaxy based web browser to perform individual and group analysis and share their results. Programming support is also available to configure and run customized pipelines. Users can view a demo of the Metabiome Database here.
ZBIT Bioinformatics Toolbox
- TFpredict: Identification and structural characterization of transcription factors
- SABINE: Prediction of the binding specificity of transcription factors
- SBML2LaTeX: Conversion of SBML files into human-readable reports
- BioPAX2SBML: Conversion of BioPAX format to SBML qual
- SBMLsqueezer: Generate kinetic rate equations for biochemical networks
- EDISA: Extracting biclusters from multiple time-series of gene expression profiles
- ModuleMaster: Finding cis-regulatory modules using promoter analysis and microarray expression data regression.
30 new papers were added to the Galaxy CiteULike Group in August. This paper may be particularly interesting to the Galaxy community:
- Richard LeDuc, Matthew Vaughn, John M Fonner, Michael Sullivan, James G Williams, Philip D Blood, James Taylor, William Barnett, "Leveraging the national cyberinfrastructure for biomedical research," in Journal of the American Medical Informatics Association (JAMIA), doi:10.1136/amiajnl-2013-002059
2014 Galaxy Training Survey
Help guide Galaxy's 2014 training efforts by taking the Galaxy Training Survey! Please take a few minutes to let us know what you would like to see offered, and where you would like training to be held.
The Galaxy is expanding! Please help it grow.
- Bioinformatics post available at University of Exeter
- PhD or postdoc position available at Laboratory of Computational Biology, University of Leuven
- The Galaxy Project is hiring software engineers and post-docs.
- Sr Bioinformatics Specialist, Tufts University, Boston MA.
- Senior Developer, Stem Cell Bioinformatics Core, Sage Bionetworks, Seattle, WA, United States
Aug 12, 2013 Galaxy Distribution
- Upgrades to Data Manager including improved installation actions
- Visualization Framework tunings, plus updates to Phyloviz, Scatterplot, and Trackster
- Workflows include new reproducibility controls and editing features
- Multiple Tool Shed enhancements, features, and tunings
- Plus additional updates to the UI, Admin and Core, and API, an important Security Fix reminder, and Bug Fixes
new: $ hg clone https://bitbucket.org/galaxy/galaxy-dist#stable upgrade: $ hg pull $ hg update release_2013.08.12
Tool Shed Contributions
- samifier: enable a nexus between proteomic and genomic analysis.
- neuma: Accurate quantification of transcriptome from RNA-Seq data by effective length normalization
- bwa_0_7_5: bwa version 0.7.5, nomore bwa aln, bwa same/sampe. BWA-MEM only for this integration version
- coverage_correlation: calculate pearson correlation of coverages. outputs clustering/correlation matrix
- chemicaltoolbox: install cheminformatic tool wrappers (chemfp, openbabel, osra, confab, ...)
ctb_machine_learning: machine learning scripts for the chemicaltoolbox
- + many others from bgruening: opsin, simsearch, rdkit, silicos_it, confab, osra, chemfp, openbabel, ...
- nammer: find rRNA genes in a DNA sequence
- unique: advanced unique lines program; filter by unique lines or a unique column subset.
- lots of SAM/BAM/Pileup tools
- bwa_tools_mini: bwa_tools with restricted resources for use in classes
- bioblend 0.4.0 is out: CloudMan and Galaxy API library
- Write up of BOSC2013, AFP13 ISMB/ECCB in GigaBlog: Bioinformaticians breaking down barriers in Berlin.
- TRON technologists have developed a laboratory information management system (LIMS) for a next-generation sequencing (NGS) laboratory within the existing Galaxy platform. Code and documentation are available for download.
- Saket Choudhary's Week 7+8 GSOC update: making Workflow's run page editable; enable user to change params at runtime
- galaxy-bootstrap - a simple Java library and download, configure, and seed a Galaxy instance
- New Cloud hub page on wiki giving a high-level overview of the several Galaxy cloud-deployment options.
- A new Teaching with Galaxy hub page on the Galaxy wiki. This is very much in a draft state but includes a fairly complete Computing Platforms for Teaching page.